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Somaclonal variation in tuber traits of potato

机译:马铃薯块茎性状的无性系变异

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About 13,000 somaclones of 17 cultivars and clones of potato were obtained from in vitro callus cultures and individually planted in a greenhouse, followed by tuber generations grown in the field. These plants were subjected to the multistage selection procedure commonly used in potato breeding. Over a period of five years and three field generations the tuber number, size, shape, eye depth, starch content, starch yield and tuber appearance of these somaclones were assessed and compared with that of the controls. These characters varied depending on donor genotype and trait. The frequency of variants was assessed and there were acceptable proportions of desirable abberrants and invariants among the somaclones. Depending on trait the average gain rate for all donor genotypes ranged between 0.2 and 2.3% for-deviants, between 12.2 and 15.5% for invariants and between 0.1–1.4% for +deviants.
机译:从体外愈伤组织培养物中获得了约13,000个17个品种的马铃薯无性克隆和马铃薯无性系,并分别种植在温室中,随后在田间种植了块茎。这些植物经过马铃薯育种中常用的多阶段选择程序。在五年的时间里,经过三个田间世代,评估了这些松果油菜的块茎数量,大小,形状,眼深,淀粉含量,淀粉产量和块茎外观,并将其与对照进行了比较。这些特征取决于供体的基因型和性状。评估了变体的频率,在体细胞克隆中有可接受比例的理想突变体和不变体。根据性状的不同,所有供体基因型的平均获得率变异范围在0.2%至2.3%之间,不变量变异范围在12.2%至15.5%之间,+变异变量则在0.1%至1.4%之间。

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