Waste Frying Oil As Substrate For Lipase Production By Geotrichum Candidum Strains

摘要

The study was carried out on 9 yeast strains of Geotrichum candidum. The purpose of the study was to determine the ability of the yeast strains to produce extra- and intracellular lipases in shake cultures in a medium containing 2% and 3% of rapeseed oil used as a carbon source, after deep frying of Frenchfries. Biosynthesis of these enzymes in an AK-210 bioreactor at a working volume of 5 L was analyzed. Lipase activity was determined using olive oil (JL_O) and glycerol tributyrate (JL_T) as substrates.rnThe results obtained in shake cultures showed that the best carbon source was the medium containing 2% of olive oil, while the best producer of extracellular lipases (198 JL_(ZO) mL~(-1)) was the strain of G. candidum MSK_3-11.rnLipase synthesis in the bioreactor was begun at the exponential phase of growth. The activity of extracellular lipases amounted to 88 JL_(ZO) mL~(-1) at biomass yield of 14 g L~(-1) at a stationary phase.rnIrrespective of the strain and the medium, both extra- and intracellular activity was higher with the use of olive oil than with tributyrin.