Transgenic Root cultures of Gentiana punctata L.

摘要

Shoot cultures of Gentiana pumctata L. were inoculated with suspension of Agrobacterium rhizogenes strain A4 M70gUS. Hairy roots which appeared 2-3 weeks later were cultured on hormone-free, liquid, WPM (Lloyd and McCown 1980) basal Medium for more than 5 years (60 subcultures). Growth rate of transformed rots was higher than the growth rate of non- Transformed roots. Spontaneous shoot regeneration occurred only in three culture vessels in subcultures No. 40 and 42. Plants Had phenotype characteristics typical for A. rhizogenes transformed plants including: wrincled leaves, short internodes, pla- Giotropic roots and in general their growth rate was reduced.