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>Dof5.6/HCA2, a Dof Transcription Factor Gene, Regulates Interfascicular Cambium Formation and Vascular Tissue Development in Arabidopsis
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Dof5.6/HCA2, a Dof Transcription Factor Gene, Regulates Interfascicular Cambium Formation and Vascular Tissue Development in Arabidopsis
n nnnVascular cambium, a type of lateral meristem, is the sourceof secondary xylem and secondary phloem, but little is knownabout the molecular mechanisms of its formation and development.Here, we report the characterization of an Arabidopsis thalianagain-of-function mutant with dramatically increased cambialactivity, designated high cambial activity2 (hca2). The hca2mutant has no alternative organization of the vascular bundles/fibersin inflorescence stems, due to precocious formation of interfascicularcambium and its subsequent cell division. The phenotype resultsfrom elevated expression of HCA2, which encodes a nuclear-localizedDNA binding with one finger (Dof) transcription factor Dof5.6.Dof5.6/HCA2 is preferentially expressed in the vasculature ofall the organs, particularly in the cambium, phloem, and interfascicularparenchyma cells of inflorescence stems. Dominant-negative analysisfurther demonstrated that both ubiquitous and in situ repressionof HCA2 activity led to disruption of interfascicular cambiumformation and development in inflorescence stems. In-depth anatomicalanalysis showed that HCA2 promotes interfascicular cambium formationat a very early stage of inflorescence stem development. Thisreport demonstrates that a transcription factor gene, HCA2,is involved in regulation of interfascicular cambium formationand vascular tissue development in Arabidopsis.展开▼
National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China;
National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China;
National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China|National Plant Gene Research Center (Beijing), Beijing 100101, People's Republic of China;
National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China|National Plant Gene Research Center (Beijing), Beijing 100101, People's Republic of China;