n nnnFloral organ identity and meristem determinacy in plants arecontrolled by combinations of activities mediated by MADS boxgenes. AGAMOUS-LIKE6 (AGL6)-like genes are MADS box genes expressedin floral tissues, but their biological functions are mostlyunknown. Here, we describe an AGL6-like gene in rice (Oryzasativa), MOSAIC FLORAL ORGANS1 (MFO1/MADS6), that regulatesfloral organ identity and floral meristem determinacy. In theflower of mfo1 mutants, the identities of palea and lodiculeare disturbed, and mosaic organs were observed. Furthermore,the determinacy of the floral meristem was lost, and extra carpelsor spikelets developed in mfo1 florets. The expression patternsof floral MADS box genes were disturbed in the mutant florets.Suppression of another rice AGL6-like gene, MADS17, caused nomorphological abnormalities in the wild-type background, butit enhanced the phenotype in the mfo1 background, indicatingthat MADS17 has a minor but redundant function with that ofMFO1. Whereas single mutants in either MFO1 or the SEPALLATA-likegene LHS1 showed moderate phenotypes, the mfo1 lhs1 double mutantshowed a severe phenotype, including the loss of spikelet meristemdeterminacy. We propose that rice AGL6-like genes help to controlfloral organ identity and the establishment and determinacyof the floral meristem redundantly with LHS1.展开▼
机译：ABSTRACTn n
n TOP n <字体颜色= 464c53>抽象 n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n n nnn植物的花器官身份和分生组织确定性是 控制由MADS box 基因介导的活动组合所引导。 AGAMOUS-LIKE6 （ AGL6 ）样基因是在花卉组织中表达的 的MADS盒基因，但其生物学功能主要是 未知。在这里，我们描述了水稻（ Oryza sativa ）， MOSLOR FLORAL ORGANS1 （ MFO1 / MADS6 ），它调节 花器官的身份和花分生组织的确定性。在 mfo1 突变体的 花中，pala and lodicule 的身份受到干扰，并观察到花叶器官。此外， 的花分生组织的确定性丢失，并且在 mfo1 小花中形成了多余的心皮 或小穗。 MADS盒花基因的表达模式 在突变小花中受到干扰。 另一个水稻 AGL6 样基因 MADS17 < / I>，在野生型背景中未引起 形态异常，但 增强了 mfo1 背景中的表型，表明 与 MFO1 相比， MADS17 具有次要但多余的功能。 MFO1 或 SEPALLATA -样 基因 LHS1 中的单个突变体表现出中等的表型，而 mfo1 lhs1 双突变体 表现出严重的表型，包括小穗分生组织 的确定性丧失。我们建议水稻 AGL6 样基因有助于控制 花器官的身份，并与 LHS1冗余地建立花分生组织的建立和确定性 。 。展开▼
Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, National Agriculture and Food Research Organization, Niigata 943-0193, Japan;
Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, National Agriculture and Food Research Organization, Niigata 943-0193, Japan;
Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;
Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan;
Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan;
Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, National Agriculture and Food Research Organization, Niigata 943-0193, Japan;
Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan;
Rice Biotechnology Research Subteam (Hokuriku Region), National Agricultural Research Center, National Agriculture and Food Research Organization, Niigata 943-0193, Japan;
