n nnnThe transcriptional regulation of the LATE ELONGATED HYPOCOTYL(LHY) gene is key to the structure of the circadian oscillator,integrating information from multiple regulatory pathways. Weidentified a minimal region of the LHY promoter that was sufficientfor rhythmic expression. Another upstream sequence was alsorequired for appropriate waveform of transcription and for maximumamplitude of oscillations under both diurnal and free-runningconditions. We showed that two classes of protein complexesinteract with a G-box and with novel 5A motifs; mutation ofthese sites reduced the amplitude of oscillation and broadenedthe peak of expression. A genome-wide bioinformatic analysisshowed that these sites were enriched in phase-specific clustersof rhythmically expressed genes. Comparative genomic analysesshowed that these motifs were conserved in orthologous promotersfrom several species. A position-specific scoring matrix forthe 5A sites suggested similarity to CArG boxes, which are recognizedby MADS box transcription factors. In support of this, the FLOWERINGLOCUS C (FLC) protein was shown to interact with the LHY promoterin planta. This suggests a mechanism by which FLC might affectcircadian period.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn 晚期加长的羟丙基 I> SU的转录调控P>( LHY I>)基因是昼夜节律振荡器结构的关键, SUP>整合了来自多个调控途径的信息。我们 SUP>确定了 LHY I>启动子的最小区域,该区域足以进行有节奏的表达。还需要另一个上游序列,以获取适当的转录波形,并在日间和自由运行的条件下均获得最大的振荡振幅。 SUP> SUP>我们发现,两类蛋白质复合物 SUP>与G-box和新颖的5A基序相互作用。这些位点的 SUP>突变降低了振荡幅度,并加宽了 SUP>表达峰。全基因组生物信息学分析 SUP>表明,这些位点富含节律性表达基因的阶段特异性簇 SUP>。比较基因组分析 SUP>表明,这些基序在几种物种的直系启动子 SUP>中是保守的。 SUP> 5A位点的位置特定评分矩阵表明与CArG盒相似,MAr盒转录因子可识别 SUP>。为此,FLOWERING SUP> LOCUS C(FLC)蛋白与植物中的 LHY I>启动子 SUP>相互作用。这表明FLC可能会影响 SUP>昼夜节律周期的机制。 SUP>
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Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom;
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom;
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom|Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom;
MRC Biostatistics Unit, Institute of Public Health, University Forvie Site, Cambridge CB2 0SR, United Kingdom;
Systems Biology Centre, University of Warwick, Coventry CV4 7AL, United Kingdom;