n nnnThe establishment and maintenance of auxin maxima in vascularplants is regulated by auxin biosynthesis and polar intercellularauxin flow. The disruption of normal auxin biosynthesis in mouse-earcress (Arabidopsis thaliana) leads to severe abnormalities,suggesting that spatiotemporal regulation of auxin biosynthesisis fundamental for normal growth and development. We have shownpreviously that the induction of the SHORT-INTERNODES/STYLISH(SHI/STY) family member STY1 results in increased transcriptlevels of the YUCCA (YUC) family member YUC4 and also higherauxin levels and auxin biosynthesis rates in Arabidopsis seedlings.We have also shown previously that SHI/STY family members redundantlyaffect development of flowers and leaves. Here, we further examinethe function of STY1 by analyzing its DNA and protein bindingproperties. Our results suggest that STY1, and most likely otherSHI/STY members, are DNA binding transcriptional activatorsthat target genes encoding proteins mediating auxin biosynthesis.This suggests that the SHI/STY family members are essentialregulators of auxin-mediated leaf and flower development. Furthermore,the lack of a shoot apical meristem in seedlings carrying afusion construct between STY1 and a repressor domain, SRDX,suggests that STY1, and other SHI/STY members, has a role inthe formation and/or maintenance of the shoot apical meristem,possibly by regulating auxin levels in the embryo.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn维管植物 SUP>中最大生长素的建立和维持生长素的生物合成和极性细胞间 SUP>生长素的流量对其进行调节。 SUP>水芹( Arabidopsis thaliana I>)正常植物生长素生物合成的破坏导致严重异常, SUP>表明植物生长素生物合成的时空调控 > SUP>是正常增长和发展的基础。我们先前已经显示 SUP>表示 SHORT-INTERNODES / STYLISH I> SUP>( SHI / STY I>)家庭成员< I> STY1 I>导致 YUCCA I>( YUC I>)家族成员 YUC4 I>的转录 SUP>水平升高,并且还提高了拟南芥 I>幼苗中的 SUP>植物生长素水平和植物生长素生物合成速率。 SUP>我们之前也已经证明了 SHI / STY I>家族成员多余地 SUP>影响花朵和叶子的发育。在这里,我们通过分析STY1的DNA和蛋白质结合 SUP>特性进一步检查 SUP>的功能。我们的结果表明,STY1和最可能的其他 SUP> SHI / STY成员是DNA结合转录激活因子 SUP>,其靶向编码介导生长素生物合成的蛋白质的基因。 SUP>这表明SHI / STY家族成员是生长素介导的叶和花发育的必需 SUP>调节剂。此外, SUP>在STY1和阻遏域SRDX, SUP>之间携带 SUP>融合构建体的幼苗中缺少茎尖分生组织,这表明 STY1 I>和其他 SHI / STY I>成员在 SUP>茎尖分生组织 SUP>的形成和/或维持中起作用可能通过调节胚胎中的生长素水平。 SUP>
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