n nnnPeroxisomes are highly dynamic organelles involved in variousmetabolic pathways. The division of peroxisomes is regulatedby factors such as the PEROXIN11 (PEX11) proteins that promoteperoxisome elongation and the dynamin-related proteins (DRPs)and FISSION1 (FIS1) proteins that function together to mediateorganelle fission. In Arabidopsis thaliana, DRP3A/DRP3B andFIS1A (BIGYIN)/FIS1B are two pairs of homologous proteins knownto function in both peroxisomal and mitochondrial division.Here, we report that DRP5B, a DRP distantly related to the DRP3sand originally identified as a chloroplast division protein,also contributes to peroxisome division. DRP5B localizes toboth peroxisomes and chloroplasts. Mutations in the DRP5B genelead to peroxisome division defects and compromised peroxisomefunctions. Using coimmunoprecipitation and bimolecular fluorescencecomplementation assays, we further demonstrate that DRP5B caninteract or form a complex with itself and with DRP3A, DRP3B,FIS1A, and most of the Arabidopsis PEX11 isoforms. Our datasuggest that, in contrast with DRP3A and DRP3B, whose orthologsexist across plant, fungal, and animal kingdoms, DRP5B is aplant/algal invention to facilitate the division of their organelles(i.e., chloroplasts and peroxisomes). In addition, our resultssupport the notion that proteins involved in the early (elongation)and late (fission) stages of peroxisome division may act cooperatively.展开▼
机译：ABSTRACTn n
n TOP n <字体颜色= 464c53>抽象 n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n n nnn过氧化物酶体是参与各种 metabo的高度动态的细胞器lic途径。过氧化物酶体的分裂受诸如促进 过氧化物酶体伸长的PEROXIN11（PEX11）蛋白和与动力蛋白有关的蛋白（DRPs） 等因素调控。和FISSION1（FIS1）蛋白一起起作用，以介导 细胞器裂变。在拟南芥中，DRP3A / DRP3B和 FIS1A（BIGYIN）/ FIS1B是两对已知的 同源蛋白，它们在过氧化物酶体和线粒体分裂中均起作用。 在这里，我们报道DRP5B是与DRP3s 密切相关的DRP，最初被鉴定为叶绿体分裂蛋白， 也有助于过氧化物酶体的分裂。 。 DRP5B定位于 过氧化物酶体和叶绿体。 DRP5B 基因 中的突变导致过氧化物酶体分裂缺陷和过氧化物酶体 功能受损。使用共免疫沉淀和双分子荧光 互补分析，我们进一步证明DRP5B可以与自身以及与DRP3A，DRP3B， FIS1A相互作用或形成复合物，和大多数拟南芥 PEX11亚型。我们的数据 建议，与直系同源物 存在于植物，真菌和动物界的DRP3A和DRP3B相反，DRP5B是 植物/藻类发明，以促进其细胞器 （即叶绿体和过氧化物酶体）的分裂。此外，我们的结果 支持以下观点，即过氧化物酶体分裂的早期（延伸） 和晚期（裂变）阶段所涉及的蛋白质可能协同作用。展开▼
Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824;
Michigan State University–Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824|Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824;
