Regional Training Workshop on Training on DNA Sequencing and TILLING, TUR/5/025, Ankara, Turkey, 26-30 April 2010

摘要

The objective of the course was to provide theoretical background and hands-on training in reverse-genetics using induced mutations and high-throughput mutation discovery methods. All steps of the TILLING procedure were covered. Topics included methods of mutation induction, population structure, DNA extraction, DNA quantification, nomenclature, sample storage, computational methods for gene identification and primer design, mutation discovery, sequencing of mutations and data analysis. A set of positive control samples developed in Seibersdorf were used for the course and students worked in teams to identify known mutations. Both low-cost aga-rose gel and high-throughput fluorescent readout platforms were used. The course was a success and induced mutations were recovered. This indicates that TILLING can be successfully performed at the Turkish Atomic Energy Authority (TAEK). From this preliminary data, a research plan was developed for further assay optimizations, and continued close ties between TAEK and the Plant Breeding and Genetics Laboratory in Seibersdorf are expected.