Ectopic expression of ARR1ΔDDK in tobacco: alteration of cell fate in root tip region and shoot organogenesis in cultured segments

摘要

A specific deleted version of ARABIDOPSIS RESPONSE REGULATOR1 (ARR1) lacking the signal receiver domain (1–152 amino acids)-coding sequence, referred to as ARR1ΔDDK, was amplified using Arabidopsis thaliana cDNA prepared from adult leaves and transferred into the genome of Nicotiana tabacum cv. Samsun under the transcriptional control of a β-estradiol-inducible expression system. The ectopic expression of ARR1ΔDDK affected the morphology of transgenic seedlings and their segments in vitro. In the presence of an inducer, β-estradiol, ectopic expression of ARR1ΔDDK induced only the formation of soft, pseudo-bulbous tissue in the root tip region of intact seedlings, which appeared similar to callus generated on a hypocotyl segment in the presence of 2,4-d and 6-benzyladenine (BA), both at 1 μM. Those callus tissues on the root tip region could not generate shoots unless 1 μM BA was supplied. In segment culture, ectopic expression of ARR1ΔDDK induced callus-like tissue around the cut-end of cotyledon and hypocotyl segments with occasional shoot formation, suggesting that the expression of ARR1ΔDDK could substitute for the effects of cytokinin on these segments. Additionally, treatment with only β-estradiol induced NtWUS, a WUS ortholog in tobacco, which was detected during the process of callus tissue formation in the root tip region and also in cotyledon or hypocotyl segments. These findings suggest that the NtWUS might be associated in the transdifferentiation process caused by the functional regulation of ARR1ΔDDK in transgenic tobacco seedlings. Keywords ARR1ΔDDK - Transdifferentiation - Root tip bulbing - NtWUS - Type-B ARR - Tobacco