Inactivation of specific spoilage organism (Pseudomonas) of sturgeon by curcumin-mediated photodynamic inactivation

摘要

The present study aimed to measure the inactivation effect and mechanism of curcumin-mediated photodynamic inactivation (PDI) on the specific spoilage organism (Pseudomonas) of the sturgeon. The conditions of PDI used were as follows: 30 mu M curcumin, 15 W LED light (470 nm) power and 90 s irradiation time. Under these conditions, the high-throughput sequencing was used to study the microbiota of sturgeon. The method of aerobic plate colony count (APC) was used to determine the viability of Pseudomonas after PDI. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the propidium iodide (PI) single staining method, and agarose gel electrophoresis were used to study the inactivation mechanism of PDI on Pseudomonas. The results showed that Pseudomonas was the specific spoilage organism of sturgeon, and PDI significantly inhibited the growth of Pseudomonas. The in-vitro inactivation rate of Pseudomonas was 99.9% with counts decreased by 3.19 +/- 0.15 log(10) CFU/mL. The mechanism of PDI to inactivate Pseudomonas is as follows. Firstly, the high-level structure of membrane protein was destroyed, and the cell membrane permeability was increased, which caused leakage of cellular content. Then the nucleic acid inside the cell was destroyed, which eventually caused the death of bacteria. These findings demonstrate that curcumin-mediated PDI can be utilized as an effective way to inactivate the specific spoilage organism (Pseudomonas) of the sturgeon.