A thiophen-thiooxorhodamine conjugate fluorescent probe for detecting mercury in aqueous media and living cells

摘要

A rhodamine-based sensor RB-S2 was designed and synthesized by combination of the thiospirolacton chromophore and the thiophen ring block with high affinity to Hg~2+. Probe RB-S2 exhibits high selectivity and excellent sensitivity in both absorbance and fluorescence detection of Hg~2+ in aqueous solution. In addition, fluorescent imaging of Hg~2+ in MCF-7 cells is also successfully demonstrated.