Portable multi-spectral lens-less microscope with wavelength-self-calibrating imaging sensor

摘要

Multi-spectral imaging technology is helpful to obtain better contrast and more details of images at different wavelengths compared with monochromatic imaging or conventional RGB imaging technologies. And in resource limited areas, microscopes with features of low-cost and portability are extremely attractive. In this manuscript, we demonstrate that cost-effective lens-less microscopy can be combined with the multi-spectral imaging technology, which can be alternative to conventional approaches and applied in biology, chemistry, environmental science. As a proof of this concept, we present a novel portable multi-spectral lens-less microscope (MSLLM). An outstanding highlight of our setup is its wavelength-self-calibrating imaging sensor (WSCIS), which can provide computationally optimized wavelength value (COWV) for LED illumination in real time. For each illumination, 5 holograms of the sample at 5 different heights are captured. Through the COWVs and phase retrieval algorithms, 7 multi-spectral images (FOV: 4.8 mm*4.6 mm, resolution: similar to 1 mu m) can be reconstructed. We believe that our MSLLM can be widely applied to multi-spectral microscopy in low-cost required scenes.