LETTER TO THE EDITOR

摘要

Dear Editor, We report a novel follow-up observation pertaining to "An in vivo patient-derived model of endogenous IDH1 -mutant glioma," which was recently published in Neuro-Oncology. Since publication, we have observed the gradual and repeated loss of the wild-type IDH1 allele in vitro with retention of the mutant allele. Sequencing of IDH1 exon 4 from 3 independent late passage cultures showed homozygosity for the R132H allele (mut/-), whereas both mutant and wild-type alleles were present in the original line (mut/wt) and tumor (Fig. 1A).