Regulatory analysis of the C. elegans genome with spatiotemporal resolution

摘要

Discovering the structure and dynamics of transcriptional regulatory events in the genome with cellular and temporal resolution is crucial to understanding the regulatory underpinnings of development and disease. We determined the genomic distribution of binding sites for 92 transcription factors and regulatory proteins across multiple stages of Caenorhabditis elegans development by performing 241 ChIP-seq (chromatin immunoprecipitation followed by sequencing) experiments. Integration of regulatory binding and cellular-resolution expression data produced a spatio-temporally resolved metazoan transcription factor binding map. Using this map, we explore developmental regulatory circuits that encode combinatorial logic at the levels of co-binding and co-expression of transcription factors, characterizing the genomic coverage and clustering of regulatory binding, the binding preferences of, and biological processes regulated by, transcription factors, the global transcription factor co-associations and genomic subdomains that suggest shared patterns of regulation, and identifying key transcription factors and transcription factor co-associations for fate specification of individual lineages and cell types.%在这篇论文中, "modENCODE consortium"对线虫发育多个阶段的92个转录因子和调控蛋白的结合点的基因组分布进行了研究。通过对这些数据和细胞分辨率的表达数据进行整合, 他们生成了后生动物转录因子结合点的一个时空分布图, 该图被用来研究发育调控线路的设计和性质。