Flavonoids inhibit the genotoxicity of hydrogen peroxide (H₂O₂) and of the food mutagen 2-amino-3-methylimadazo[4,5-f]-quinoline (IQ) in lymphocytes from patients with inflammatory bowel disease (IBD)

摘要

Inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC) is a chronic inflammatory gastrointestinal autoimmune condition with an inappropriate immune response. We investigated DNA damage induced in vitro in lymphocytes from IBD patients caused by oxidative stress through H₂O₂ and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and whether the plant flavonoids, quercetin and epicatechin, found in fruits, tea and soybeans could effectively reduce such stress. Lymphocytes from IBD patients and healthy volunteers were treated with 50 μg/ml H₂O₂ or IQ in the presence of quercetin (0–250 μg/ml) or epicatechin (0–100 μg/ml). Flavonoid supplementation (250 μM quercetin or 100 μM epicatechin) caused an overall significant decrease of induced DNA damage resulting in a 48.6% (P 0.001) reduction of H₂O₂-induced and a 43% (P 0.001) reduction of IQ-induced DNA damage within the patient groups; for the control groups, reductions in DNA damage were 35.2 and 57.1%, respectively (both, P 0.001). There was less induced DNA damage within lymphocytes from UC patients compared to CD patients for both series of experiments (H₂O₂ and quercetin, IQ and epicatechin). In conclusion, flavonoids dramatically reduced oxidative stress in vitro in lymphocytes from IBD patients and healthy individuals. Thus, flavonoids could be very effective in the treatment of oxidative stress and encouraged in the diet of IBD patients.