首页> 外文期刊>Molecular Plant >Overproduction of Alkaloid Phytoalexins in California Poppy Cells Is Associated with the Co-Expression of Biosynthetic and Stress-Protective Enzymes
【24h】

Overproduction of Alkaloid Phytoalexins in California Poppy Cells Is Associated with the Co-Expression of Biosynthetic and Stress-Protective Enzymes

机译:加利福尼亚罂粟细胞中生物碱植物抗毒素的过量生产与生物合成和胁迫保护酶的共表达相关

获取原文
获取原文并翻译 | 示例
       

摘要

Many plant cells respond to pathogens by the induction of phytoalexin biosynthesis, but the underlying changes of gene expression are often obscured by their close linkage to the complex rearrangements during pathogen defense, especially the hypersensitive cell death. In root-derived cell cultures of Eschscholzia californica, the overproduction of cytotoxic benzophenanthridine alkaloids can be triggered by a minimum of pathogen pressure that does not evoke hypersensitive reactions. Such conditions activate a signal chain that is initiated by a short contact to low concentrations of yeast glycoprotein elicitor and includes a transient acidification of the cytoplasm. In contrast, high elicitor concentrations signal via an increase of jasmonate and trigger hypersensitive cell death, preceded by a drastic decay of translatable mRNAs. The main changes in protein and mRNA patterns caused by either signal path were compared by 2D proteomic separation, MS/MS sequencing and mRNA- vitro translation. The four proteins showing the highest overexpression were identical between cells that received low or high-elicitor treatment and overlapped with the three proteins most up-regulated by artificial pH shifts. They comprised one biosynthetic enzyme (norcoclaurine:SAM 4' O-methyl-transferase) plus a unique combination of stress-protective proteins: a heat shock protein (hsp 70); a peptidyl-prolyl-c/s/trans isomerase (cyclophilin); and a glyceraldehyde-3-phosphate dehydrogenase. It appears that overproduction of the benzophenanthridine phytoalexins requires the up-regulation of a rate-limiting biosynthetic enzyme plus the coordinated expression of a specific set of protective enzymes and thus is managed like an oxidative stress.
机译:许多植物细胞通过诱导植物抗毒素生物合成来对病原体作出反应,但是基因表达的潜在变化通常因其与病原体防御过程中复杂的重排紧密联系而被掩盖,尤其是超敏细胞死亡。在加州Eschscholzia californica的根源细胞培养物中,最小的病原体压力不会引起过敏反应,从而触发细胞毒性的苯并菲啶生物碱的过量生产。这样的条件激活了信号链,该信号链通过与低浓度的酵母糖蛋白引发剂短时间接触而启动,并包括细胞质的短暂酸化。相比之下,高激发子浓度通过茉莉酸酯的增加发出信号并触发超敏细胞死亡,随后可翻译的mRNA急剧衰减。通过2D蛋白质组学分离,MS / MS测序和mRNA- / n体外翻译比较了由任一信号路径引起的蛋白质和mRNA模式的主要变化。表现出最高过表达的四种蛋白质在接受了低或高激发子处理的细胞之间是相同的,并且与通过人工pH改变上调的三种蛋白质重叠。它们包含一种生物合成酶(降尿酸嘌呤:SAM 4'O-甲基转移酶),加上独特的应激保护蛋白组合:热激蛋白(hsp 70);肽基-脯氨酰-c / s /反式异构酶(亲环蛋白);和3-磷酸甘油醛脱氢酶。看起来,苯甲菲啶植物抗毒素的过量生产需要限速生物合成酶的上调加上一组特定保护酶的协同表达,因此可以像氧化应激一样进行管理。

著录项

  • 来源
    《Molecular Plant》 |2010年第5期|p.927-939|共13页
  • 作者单位

    Kliment-Ohridski-University of Sofia, Faculty of Biology, Institute of Molecular Biology, Sofia, Bulgaria;

    Martin-Luther-University of Halle-Wittenberg, Institute of Pharmacy, Department of Pharmaceutical Biology and Pharmacology, Laboratory of Molecular Cell Biology, Kurt Mothes Str. 3, 06120 Halle (Saale), Germany;

    Martin-Luther-University of Halle-Wittenberg, Institute of Pharmacy, Department of Pharmaceutical Biology and Pharmacology, Laboratory of Molecular Cell Biology, Kurt Mothes Str. 3, 06120 Halle (Saale), Germany;

    Max-Planck-Research Group 'Enzymatics of Protein Folding', Laboratory of Mass Spectrometry, Halle (Saale), Germany;

    Martin-Luther-University of Halle-Wittenberg, Institute of Pharmacy, Department of Pharmaceutical Biology and Pharmacology, Laboratory of Molecular Cell Biology, Kurt Mothes Str. 3, 06120 Halle (Saale), Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    benzophenanthridine alkaloid biosynthesis; signal transduction; stress protection; functional proteomics; ph signaling;

    机译:苯并菲啶生物碱的生物合成;信号转导;压力保护;功能蛋白质组学;ph信号;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号