Immunohistochemical retrieval of the principal HIV antigens p 24, gp 41, and gp 120 in formalin Fixed tissue: an investigation suing HIV infected Lymphoblasts and postmortem brain tissue form AIDS cases

摘要

This paper describes the use of an auto- claving procedure followed by immuno- cytochemistry to enhance the detection of the human immunodeficiency virus (HIV) antigens p24, gp41, and gp 120. This proce- dure greatly improved the detection rate of the p24 and gp41 HIV surface antigens in formalin fixed, paraffin wax embedded, HIV positive central nervous system (CNS) tissue while restricting staining to areas of the CNS showing evidence of neu- ropathology.