Vaccines comprising HIV gp120 envelope polypeptides from vaccinees vaccinated with an earlier HIV gp120 vaccine and subsequently infected with HIV

摘要

An isolated HIV envelope polypeptide comprising a HIV gp120 amino acid sequence selected from the group consisting of sequence(s) disclosed and fragments thereof, wherein each of the fragments comprises the V2, V3 and C4 domains of gp120. An immunogenic composition comprising: (a) A polypeptide comprising a first gp120 amino acid sequence or fragment thereof, wherein the first HIV gp120 is gp120 MN, gp120 A244, gp120 MN-GNE6 or gp120 MNGNE8; and (b) A polypeptide comprising a breakthrough isolate gp120 amino acid sequence or a fragment thereof, wherein the breakthrough isolate gp120 amino acid sequence is derived from a vaccinee vaccinated with the first polypeptide; Wherein the fragment comprises at least the V2, V3 and C4 domains of gp120 and the polypeptides are in a suitable carrier. The immunogenic composition is useful as an HIV vaccine.