Identification of Aluminum-Regulated Genes by cDNA-AFLP Analysis of Roots in Two Contrasting Genotypes of Highbush Blueberry (Vaccinium corymbosum L.)

摘要

To investigate the molecular mechanisms of Al3+-stress in blueberry, a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify Al-regulated genes in roots of contrasting genotypes of highbush blueberry (Brigitta, Al3+-resistant and Bluegold, Al3+-sensitive). Plants grown in hydroponic culture were treated with 0 and 100 μM Al3+ and collected at different times over 48 h. Seventy transcript-derived fragments (TDFs) were identified as being Al3+ responsive, 31 of which showed significant homology to genes with known or putative functions. Twelve TDFs were homologous to uncharacterized genes and 27 did not have significant matches. The expression pattern of several of the genes with known functions in other species was confirmed by quantitative relative real-time RT-PCR. Twelve genes of known or putative function were related to cellular metabolism, nine associated to stress responses and other transcription and transport facilitation processes. Genes involved in signal transduction, photosynthetic and energy processes were also identified, suggesting that a multitude of processes are implicated in the Al3+-stress response as reported previously for other species. The Al3+-stress response genes identified in this study could be involved in Al3+-resistance in woody plants.