Human Insulin Genome Sequence Map, Biochemical Structure of Insulin for Recombinant DNA Insulin

摘要

Abstract: Insulin is a essential molecule for type I diabetes that is marketed by very few companies. It is thenfirst molecule, which was made by recombinant technology; but the commercialization process is veryndifficult. Knowledge about biochemical structure of insulin and human insulin genome sequence map isnpivotal to large scale manufacturing of recombinant DNA Insulin. This paper reviews human insulin genomensequence map, the amino acid sequence of porcine insulin, crystal structure of porcine insulin, insulinnmonomer, aggregation surfaces of insulin, conformational variation in the insulin monomer, insulin X-raynstructures for recombinant DNA technology in the synthesis of human insulin in Escherichia coli.