Interaction of C_(60) fullerene complexed to doxorubicin with model bilipid membranes and its uptake by HeLa cells

摘要

With an aim to elucidate the effects of C_(60) fullerene complexed with antibiotic doxorubicin (Dox) on model bilipid membranes (BLM), the investigation of the electrical properties of BLM under the action of Dox and C_(60) fullerene, and of their complex, C_(60) + Dox, was performed. The complex as well as its components exert a clearly detectable influence on BLM, which is concentration-dependent and also depends on phospholipid composition. The mechanism of this effect originates either from intermolecular interaction of the drug with fatty-acid residues of phospholipids, or from membranotropic effects of the drug-induced lipid peroxidation, or from the sum of these two effects. By fluorescence microscopy the entering of C_(60) + Dox complex into HeLa cells was directly shown.