Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering

摘要

A silk ftbroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, cross-linking, and lyophilizing processes. We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility. The optimal ratio of SF/COL/HA scaffold was 3:6.5:0.5. Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied. PAP-SF/COL/HA scaffold had favorable adhesion and proliferation. A rabbit cartilage defect model was established. The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups. The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.