Comparative Time-Course of Benzo[a] pyrene-DNA Adduct Formation, and its Relationship to CYP1A Activity in Two Species of Catfish

摘要

Studies have demonstrated differences between the brown bullhead (Ameriurus nebulosus)(BB) and a closely related species of catfish, the channel catfish (Lctalurus punctatus)(CC) in activity of important liver enzymes that may affect their ability to activate or detoxify certain procarcinogens. We have mea- sured the formation and persistence of benzo[a]pyrene-DNA adducts in the liver of these two species using the ~32P-postlabeling (PPL) method, along with the effect of prior induction by β-naphthoflavone (βNF).