Calcin Accumulation in Mussel Blood Cells

摘要

Incubation of mussel blood cells with the lipophylic compound calcein-AM led these cells to hydrolyse this organic chemical into its charged and fluorescent from, calcein. The distribution of this dye was mainly concern with the lysosomal compartment, as visualized by confoncal laser microscopy. Assessment of blood cell calcein accumulation with a microtiter plate reader showed that calcein fluorescence was lower in mussel cells from organisms collected at a polluted site and from organisms treated with vincristine than in cells from organisms sampled in a pristine area.