Preparation of a porous scaffold based on polypropylene grafted with monomethylitaconate as potential bone graft

摘要

Based on polypropylene (PP) grafted with different percentages of maleic anhydride (PP-g-MA) or monomethylitaconate (PP-g-MMI) a new porous scaffold was prepared with porosities in the range of 50–200 μm. The swelling capacity was analyzed, and morphological, mechanical, and elemental analyses of these scaffolds were carried out. In vitro swelling in the simulated body fluid (SBF), chitosan (CHI) of low (70 kDa) and high (350 kDa) molecular weight, and chitosan-hydroxyapatite solutions were assayed at 37 °C from 24 h to 4 weeks. The swelling degree (SD) of these scaffolds was in the range of 25%–125%. The highest SD value was found in the low-molecular weight (LMW) chitosan solution. The PP-g-MMI and PP-g-MA with 0.7% and 1% of grafting, respectively, showed the highest SD values in the CHI solution. The in vitro treatment of the scaffold was performed by immersion in LMW chitosan and/or the double ionic diffusion (DID) method. The pore structure of the scaffolds was unaltered after these treatments, as revealed by scanning electron microscopy (SEM). Mechanical properties, that is, fracture resistance and deformation of the porous scaffolds depended on the percentage of grafting. Scaffolds with a smaller pore size showed higher mechanical properties. Energy Dispersive Spectroscopy (EDS) measurements of PP-g-MMI with 0.7% of grafting after in vitro treatment revealed the formation of hydroxyapatite (HA) crystals with different morphologies on the porous scaffold. It was concluded that the porous scaffold based on PP-g-MMI could be used as a potential prototype bone graft.