Lipid Domain Pixelation Patterns Imposed by E-beam Fabricated Substrates

摘要

This work describes a technique for formingnnanometer-scale pixilated lipid domains that are self-organizedninto geometric patterns residing on a square lattice. In thisnprocess, a lipid multibilayer stack is deposited onto a silicansubstrate patterned with a square lattice array of bumps,nhemispherical on their sides, formed by electron beamnlithography. Domain patterns are shown to be confined tonthe flat grid between the bumps and composed of connectednand individual domain pixels. Analysis of lattices of varyingnsizes shows that domain pattern formation is driven bynmechanical energy minimization and packing constraints. Wendemonstrate single lattice sizes and a gradient in lattice sizenvarying from the micrometer to the 100 nm scale applicable to precise arraying, patterning, and transport of biomolecules thatnpartition to lipid domains.