Extraction and purification of eupatilin from Artemisia princeps PAMPAN recycling preparative HPLC

摘要

A recycling preparative HPLC was used to extract and separate the eupatilin contained in Artemisia princeps PAMPAN, and the optimum operating condition was experimentally determined. Eupatilin was extracted by ethanol solvent from the leaf and trunk of Artemisia princeps PAMPAN. The resulting solution was further partitioned with n-hexane, chloroform and ethyl acetate. The solution containing eupatilin was collected using a preparative as well as analytical column, and it was identified by LC-CE-MS. In the experiment, the mobile phase consisted of water/aceto-nitrile/TFA=50/50/0.5 (vol%), and UV wavelength measured was 370 nm. For analytical chromatography, the injection volume was 20 μL and the flow rate was fixed at 1.0 mL/min. The measured retention time for eupatilin was approximately 8.6 min in the above operating condition. For recycling preparative HPLC with commercially available GSA 310A column at 1.5 mL/min of the flow rate and 2 mL of injection volume, the purity of the eupatilin was almost 100% after recycling twice.