The construction of hpv16-L1 recombinant plasmid and its expression in mammallan cells

摘要

Infection with high risk human papillomavirus is regarded as the amjor risk factor in the development of cervical cancer. In this study, HPV16-L1 eukaryotic expression plasmids pcDNA-Li were constructed, which were transfected into mammalian cells Cos-7. The expression of HPV16-L1 in transfected cells were identified by in situ hybridization, immunospot and immunocytochemistry.