Mechanism of Adenylate Kinase. 20. Probing the Importance of the Aromaticity in Tyrosine-95 and the Ring Size in Proline-17 with Unnatural Amino Acids

摘要

We report an application of the unnatural amino acid mutagenesis developed by Schultz to probe the importance of the aromaticity of tyrosine-95 and the ring size of proline-17 in the function of adenylate kinase (AK, from chicken muscle, overexpressed in Escherichia coli).