Two-Dimensional Diversity: Screening Human cDNA Phage Display Libraries with a Random Diversity Probe for the Display Cloning of Phosphotyrosine Binding Domains

摘要

Interaction domains govern many of the protein/protein interactions that are crucial for normal cellular function. Src homology 2 (SH2) domains represent a major class of interaction domains that are responsible for the recognition of phosphotyrosine (pTyr)-containing proteins, an interaction found almost exclusively in metazoan cellular signal transduction. Complete knowledge of all possible interactions between SH2 domains and potential pTyr ligands is fundamentally important for the understanding of signal transduction itself, since any single molecular interaction can alter cellular processes.