Competition between Receptors in Dynamic Combinatorial Libraries:Amplification of the Fittest?

摘要

The strong binders survive and are amplified,while the weak perish.This principle is the foundation of dynamic combinatorial chemistry.In dynamic combinatorial libraries (DCLs),all constituents are in equilibrium through reversible reactions that exchange the building blocks from which the different library members are assembled.Adding to a DCL a "template" which binds to a subset of the library members causes the equilibrium to shift,increasing the amount of strong binders at the expense of the weak binders.Ideally,the "fittest" will be most amplified,or,in more general terms,the efficiency of amplification will correlate with the strength of binding.However,recent theoretical studies by Severin and ourselves suggest that,while the correlation between binding efficiency and amplification is often satisfactory,special cases occur where the correlation breaks down.