Photoinduced Signal Amplification through Controlled Externally Sensitized Fragmentation in Masked Sensitizers

摘要

Detection of molecular recognition events has always been an area of primary focus in bioanalytical sciences,with methods based on fluorescence being of preference due to their sensitivity.However,high throughput fluorescence binding assays require the tested ligands to be either spatially addressable or segregated on support beads for mechanical sorting.Solution-phase libraries,either unsupported or immobilized on submicrometer-sized carriers,present a challenge,as there were no direct methods to assay them.