2-Hydroxyl Proton Positions in Helical RNA from Simultaneously Measured Heteronuclear Scalar Couplings and NOEs

摘要

The ribose 2'-hydroxyl group in RNA is largely responsible for differences in structure,hydration,and thermodynamic stability relative to DNA.In addition to promoting the C3'-endo pucker of the ribose,the 2'OH group provides a scaffold for the water network in the minor groove of RNA~2 and plays a role in RNA catalysis.