Direct Adsorption and Detection of Proteins, Including Ferritin, onto Microlens Array Patterned Bioarrays

摘要

A variety of methods have been described for preparing protein arrays, including photolithography, microcontact printing, microspotting, pin spotting, and microfluidics. These methods allow different substrate properties and attachment chemistries. However, many of the arrays that have been described cannot be made in an industrially viable manner. For real world use, a protein array would be simple and inexpensive to manufacture, its fabrication amenable to automation, the size and shape of the features could be controlled, and the resulting arrays could be used for high throughput and rapid analyses.