Electrical Detection of TATA Binding Protein at DNA-Modified Microelectrodes

摘要

Sensitive methods to detect the specific binding of proteins to DNA are essential in monitoring pathways for gene expression and in the development of new diagnostics of disease states. There are several high-throughput, array-based methodologies currently available for monitoring transcription factor binding to DNA including immunoprecipitation of reversibly crosslinked chromatin/ protein complexes, capture of fluorescent antibodies by epitope-tagged DNA-binding proteins, and amplification of sequences methylated by enzyme/DNA adenine-methyltransferase fusions. However, these methods rely on fluorescence detection and require DNA amplification and/or protein labeling.