In Situ Preparation And Modification Of Supported Lipid Layers By Lipid Transfer From Vesicles Studied By Qcm-d And Tof-sims

摘要

The study of lipid transfer between lipid membranes is of high interest for the fundamental understanding of this complex and important process and, furthermore, for providing a new avenue for the in situ modification of supported lipid bilayers (SLBs). SLBs of some (but not all) compositions are conveniently formed by vesicle spreading onto a solid support. However, this method is limited to conditions (i.e., combination of vesicle lipid composition, surface chemical properties, and buffer) such that the vesicles break spontaneously upon adsorption to the surface and also fuse to a coherent bilayer. Many SLB compositions are therefore not accessible by this approach. In the present study, we demonstrate how this limitation can be circumvented by using lipid transfer between a preformed SLB and vesicles added in bulk to form lipid layers with new compositions and striking new features, notably with respect to stability.