PhnY and PhnZ Comprise a New Oxidative Pathway for Enzymatic Cleavage of a Carbon-Phosphorus Bond

摘要

The sequential activities of PhnY, an α-ketoglutarate/Fe(Ⅱ)-dependent dioxygenase, and PhnZ, a Fe(Ⅱ)-dependent enzyme of the histidine-aspartate motif hydrolase family, cleave the carbon-phosphorus bond of the organophosphonate natural product 2-aminoethyl-phosphonic acid. PhnY adds a hydroxyl group to the α-carbon, yielding 2-amino-l-hydroxyethylphosphonic acid, which is oxidatively converted by PhnZ to inorganic phosphate and glycine. The PhnZ reaction represents a new enzyme mechanism for metabolic cleavage of a carbon-phosphorus bond.