Sequence-Selective Detection of Double-Stranded DNA Sequences Using Pyrrole-lmidazole Polyamide Microarrays

摘要

We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imida-zole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These poly-amide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonudeotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch.