The influence on biosorption potentials of metal-resistant bacteria Enterobacter sp. EG16 and Bacillus subtilis DBM by typical red soil minerals

摘要

Purpose Due to the inevitable interaction between bacteria and soil minerals, whether bacteria could exert the excepted functions in the soil is yet to be confirmed and how minerals affect biosorption potential is needed to be investigated. The purposes of this study were to explore the adsorption behavior and mechanism of metal-resistant bacteria attaching to typical red soil minerals under different conditions and to discuss whether biosorption potential would be altered after the addition of functional bacteria to soil. Materials and methods Here, we tested equilibrium adsorption along with zeta potential analysis, scanning electron microscopy (SEM), Fourier transform infrared spectra (FTIR), and desorption to investigate the adsorption of two metal-resistant bacteria (Gram-negative Enterobacter sp. EG16 and Gram-positive Bacillus subtilis DBM) onto typical red soil minerals including goethite, kaolinite, and gibbsite under different environmental factors. Results and discussion We found that the minerals adsorbed more EG16 cells than DBM, and the adsorption capacities followed the sequence of goethite kaolinite gibbsite. Both the surfaces of bacteria and mineral were pH-dependent in our tested pH range (4.0-7.0), and the maximum adsorption was at pH 4.0. Increasing ionic strength resulted in less adsorption of bacteria onto goethite, whereas bacterial adsorption onto kaolinite was the opposite. These observations elucidated that electrostatic interaction was the dominant contributor. The adsorption conformed to the Langmuir and pseudo-second-order kinetic model implying chemical adsorption, and the result of FTIR also supported that. Desorption experiment has suggested the significant contribution of electrostatic force and the minor dominator of functional groups for bacteria-mineral combination. Conclusions The results of this study indicated that electrostatic interaction was the dominant contributor to bacteria-mineral combination and functional groups coordination contributed less than 10%. This finding suggested most adhered bacteria could still provide active sites for heavy metal biosorption. Thus, although 50-90% of added functional bacteria has adhered to minerals, the bacteria-mineral combination had a limited impact on biosorption.