Stabilization of Collagen by Cross-linking with a Ferrous-gluconic Acid Compound

摘要

The stabilization of type I collagen is important to applications of collagen protein. In this paper, the stabilization of type I collagen with Fe-gluconic acid has been studied by various methods such as UV-vis and IR spectra, CD and DSC etc. Gluconic acid can form a compound with ferrous sulphate which can improve the thermal stability of type I collagen to an extent greater than that of Fe alone. The reaction molar ratio was 1:3 between Fe and gluconic acid. The increase of thermal stability is owed to cross-link formation between the compound and collagen.