首页> 外文期刊>Journal of Shanghai Second Medical University >Effects of tgf-β_1 on cellular proliferation and expression of type III collagen by cultured rat renal interstitial fibroblasts
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Effects of tgf-β_1 on cellular proliferation and expression of type III collagen by cultured rat renal interstitial fibroblasts

机译:tgf-β_1对培养的大鼠肾间质成纤维细胞增殖和III型胶原表达的影响

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摘要

Objective To investigate the effects of TGF-β_1 on proliferation and type III collagen mRNA expression of rat renal interstitial fibroblasts in vitro for elucidating the role of fibroblasts in renal interstitial fibrosis. Methods The cells were cultured in media containing various concentrations of TGF-β_1. The proliferation and the type III collagen mRNA expression of the cells were assayed with MTT method and RT-PCR respectively.
机译:目的探讨TGF-β_1对大鼠肾间质成纤维细胞增殖和Ⅲ型胶原mRNA表达的影响,以阐明成纤维细胞在肾间质纤维化中的作用。方法在含有不同浓度TGF-β_1的培养基中培养细胞。 MTT法和RT-PCR分别检测细胞的增殖和III型胶原mRNA的表达。

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