OVER-EXPRESSION OF A TRUNCATED TYPE Ⅱ TGF-β RECEPTOR IN NORMAL DERMAL FIBROBLASTS DECREASES TGF-β_1 AUTOPRODUCTION

摘要

Objective To determine over-expression of a truncated type Ⅱ TGF-β receptor in down-regulating TGF-β_1 auto production in normal dermal fibroblasts. Methods In vitro cultured dermal fibro-blasts were treated with rhTGF-β_1 (5ng/ml) or recombinant adenovirus containing a truncated type Ⅱ TGF-β receptor gene (50 pfu/cell). Their effects on regulating gene expression of TGF-β_1, were observed with Northern Blot. Results rh TGF-β_1 up-regulated the gene expression of TGF-β_1 ( 34% ～ 150% ) and type Ⅰ pro-collagent 13%～190%). Over-expression of a truncated receptor Ⅱ decreased the gene expression of TGF-β_1 (53%～66%). Conclusion Over-expression of the truncated TGF-β receptor Ⅱ down-regulated TGF-β_1 autoproduction via blocking signal transduction of TGF-β. This study may provide a new strategy for scar gene therapy.