Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu's reagent

摘要

A rapid and simple Spectrophotometric procedure is described for the determination of ajmaiine and brucine. The method is based on the development of blue coloured product due to reduction of tungstate and or molybdate in Folin Ciocalteu's reagent (FCR) by ajmaiine and brucine in alkaline medium. The colour is stable for more than 48 h. The chromogenic reaction has λ_(max) at 540 nm with molar absorptivity 1.64 x 10~4 and 237 x 10~3 1 mol~(-1) cm~(-1) in the Beer's law range 1-8 μg ml~(-1) and 10-100 μg ml~(-1) for ajmaiine and brucine, respectively.