Bioactivity Assessment of potentially active Actinomycetes from Rhizospheric Soil

摘要

Soil organisms have been serving as therapeutic potent components as they produce many bioactive natural products or secondary metabolites which act as antibiotics to fight against the evolving present day drug resistance along with their other important such as anti-microbial, anti-oxidant, anti-inflammatory, anti-androgenic and anticancer properties. In the present study a total of 65 actinomycetes were isolated from rhizospheric soil samples from four different locations of Rajasthan on Actinomycetes Isolation Agar (AIA) medium. Extraction of compounds using four solvents such as benzene, petroleum ether, chloroform and ethyl acetate were carried out, followed by partial purification of compounds using Thin Layer Chromatography (TLC). GC-MS technique uncovered the presence of many compounds having antimicrobial activity like antibacterial, antifungal, antioxidant, and many other activities. The potential of AIA26 isolate was tested for their antimicrobial activity in terms of inhibition zones (IZ) on 5 different indicator pathogens. It was observed that Benzene extract was active against S. aureus (IZ-24 mm) & P. aeruginosa (IZ-42 mm) and that of Chloroform extract was active against P. aeruginosa (IZ-18mm). No activity was recorded against K. pneumonia, P. vulgaris, and 5. subtilis. The results of this study suggest that rhizospheric soil of Rajasthan is a potential source and reservoir of novel antibiotics.