Evaluation of antibacterial activity and identification of bioactive metabolites by GCMS technique from Rhizospheric Actinomycetes

摘要

Since thousands of years, plants are used as a source for medicine as they are established to possess a reservoir ofbioactive compounds. Plants have been considered a great natural source of medicinal properties and can be used as either inthe form of a pure bioactive compound or as traditional preparations. Plants and soil micro-organisms both have beenconsidered as a precursor of therapeutically helpful metabolites because of their natural production of various bioactivesecondary metabolites. Evolving drug resistance these days increase the need to isolate new drugs for the treatment of manydiseases caused by multidrug-resistant organisms. To combat these multidrug-resistant pathogens, studies were carried outto isolate antibiotic compounds from actinomycetes. In the present study, a total of 65 isolates were isolated fromrhizospheric actinomycetes. Isolation, characterization and biochemical tests of actinomycetes were carried out andsubjected to fermentation and solvent extraction by four solvents as- benzene, pet ether, ethyl acetate, and chloroform ofAIA26 isolate. After extraction crude obtained was checked for their antimicrobial activity on Mueller Hinton agar (MHA)media against five indicator organisms but, activity was recorded against S. aureus and P. aeruginosa, and no activity wasobtained against P. vulgaris, K. pneumonia, and B. subtilis. By the help of GCMS technique, major compounds present havebeen identified in AIA26 isolate.