Suppressed Leaf Senescence in Chrysanthemum Transformed with a Mutated Ethylene Receptor GenemDG-ERS1(etr1-4)

摘要

Previously, Narumi et al. (2005) generated chrysanthemum plants transformed with a mutated ethylene receptor gene (mDG-ERS1(etr1-4<), and showed that thein vitro plantlets of the transformants grown aseptically in a small plastic container had a reduced sensitivity to ethylene resulting in reduced leaf yellowing after exposure to exogenous ethylene. In the present study we evaluated ethylene sensitivity of the transformants using soil-grown mature plants. When the shoots detached from soil-grown plants were treated with exogenous ethylene under continuous light, leaf yellowing (senescence) was delayed in the transformants as compared with the non-transformed plants. Furthermore, when the detached shoots were kept in darkness without ethylene treatment, the transformants showed reduced senescence as compared with those of the non-transformed plants. These results demonstrated that the mutated ethylene receptor genemDG-ERS1(etr1-4) could confer reduced sensitivity to ethylene in the leaves of mature chrysanthemum plants. This gene may be useful to generate transgenicCompositae vegetables with leaves green for a longer time and thus having a longer shelf life.