Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin

摘要

Kanamycin was used to screen T_0 seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg-L~(-1) kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is e-conomic and effective in preliminary screening the transgenic plants.