Primary Study on Experiment of Tetraploid Induction in Haliotis discus hannai with PEG

摘要

The experiment was performed on induction tetraploid of Haliotis discus hannai at two-cell stage through cell fusion with PEG treatment. In this paper, the orthogonal experiment of three factors and three levels [L_9 (3~4)] was used. Three factors andthree levels were molecular weight of PEG: 8 000, 6 000, 4 000 mol -g~(-1); PEG concentration: 45%, 50%, 55%; treatment duration time: 1, 2, 3 min, respectively. The results showed that the optimal pattern of three factors and three levels on inducing tetraploid of Haliotis discus hannai at two-cell stage through using PEG treatment were: molecular weight 4 000, concentration 55%, treatment duration time: 2 min. The highest tetraploid induction rate was 10.8% at embryo period. The three factors treatment sequence was treatment duration time —>concentration—>molecular weight.