Experimental Study on ANF Gene Transfer for Pre - vention of Intima Proliferation after Artery Injury

摘要

To detect whether the ANF gene transferring into the injured carotid arteri - al wall is able to prevent intimal hyperplasia after carotid injury. Methods A full - length ANF cD- NA was amplified from human embryo brain by reverse transcription - polymerase chain reaction (RT- PCR). It was cloned into plasmid pcDNA_3 to form the pcDNA_3/ANF construct. An inflated balloon (4.0 mm) at 4-6 ATM were drawn forth and back in left common carotid for 3-4 times in 30 New Zealand white rabbits for making carotid in jury model.